The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

The Elg1-RFC Clamp-Loading Complex Performs a Role in

Rfc1-RFC is the only essential RFC complex and opens PCNA rings for loading onto RNA-primed DNA junctions during DNA replication or DNA repair. Ctf18-RFC also catalyzes PCNA loading/unloading, but CTF18 is not essential for cell viability [4–11]. A physical interaction between Elg1p and PCNA suggest that Elg1-RFC may also open and thus load

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The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a …

The PCNA (proliferating cell nuclear antigen) ring plays central roles during DNA replication and repair. The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA during DNA damage.

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The Elg1 Replication Factor C-like Complex Functions in

Mar 14, 2013 · The ring-shaped complex PCNA coordinates DNA replication, encircling DNA to act as a polymerase clamp and a sliding platform to recruit other replication proteins. PCNA is loaded onto DNA by replication factor C, but it has been unknown how PCNA is removed from DNA when Okazaki fragments are completed or the replication fork terminates. Here we show that the …

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ELG1, a yeast gene required for genome stability, forms a

The Ctf18-RFC complex has recently been implicated in the Mrc1 dependent DNA replication checkpoint [35], while the Elg1-RFC clamp loader plays a role in DNA replication and has a redundant role

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ELG1-RFC complex | SGD

Complex: ELG1-RFC complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific …

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The Elg1 Replication Factor C-like Complex Functions in

Apr 25, 2013 · PCNA is loaded onto DNA by replication factor C, but it has been unknown how PCNA is removed from DNA when Okazaki fragments are completed or the replication fork terminates. Here we show that the Elg1 replication factor C-like complex (Elg1-RLC) functions in PCNA unloading.

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Alternative replication factor C protein, Elg1, maintains

other RFC complexes, the Elg1-containing complex plays an important role in maintaining genomic sta-bility by regulating PCNA unloading. Results Chromatin-bound PCNA levels are increased in Elg1-depleted cells In our analyses to determine which PCNA loaders are required for chromatin binding of Cdt2 and for PCNA-dependent Cdt1 degradation by

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structure–function analysis of the yeast Elg1 protein

PCNA enhances the processivity of the replicative polymerases and provides a landing platform for other proteins and enzymes. The loading of the clamp onto DNA is performed by the Replication Factor C (RFC) complex, whereas its unloading can be carried out by an RFC-like complex containing Elg1.

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The Elg1 Replication Factor C-like Complex Functions in

Aug 15, 2013 · The budding yeast Elg1 protein, the major subunit of a replication factor C-like complex, is important for genome stability, since cells lacking Elg1 exhibit increased recombination and chromosomal rearrangements.

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Elg1 Forms an Alternative PCNA-Interacting RFC Complex

Sep 16, 2003 · The canonical RFC complex and the human RFC(Ctf18) complex both bind PCNA 22, 23, whereas RFC(Rad24) loads a specialized clamp, the Liming clamp, composed of Mec3, Rad17, and Ddc1, to sites of DNA damage 24, 25. To test whether RFC(Elg1) may be involved in regulating PCNA or Liming function, we coexpressed epitope-tagged Pol30 (PCNA) or Mec3 in

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A role for the yeast PCNA unloader Elg1 in eliciting the

Jul 22, 2019 · When the DNA is damaged, or when its faithful duplication is delayed by DNA polymerase stalling, the cells induce a coordinated response termed the DNA damage response (DDR) or checkpoint. Elg1 forms an RFC-like complex in charge of unloading the DNA polymerase processively factor PCNA during DNA replication and DNA repair.

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Eukaryotic clamp loaders and unloaders in the maintenance

Dec 18, 2020 · In eukaryotes, all five subunits of the RFC complex are essential for cell survival 33,64.The other large subunits of RLCs, Rad24 (Rad17 in fission yeast and humans), Ctf18, and Elg1 (ATAD5 in

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Regulation of Elg1 activity by phosphorylation

Jul 15, 2015 · Elg1 shares sequence homology with Rfc1 and interacts with the 4 small subunits of RFC to create an RFC-like complex (RLC). 13-15 The Elg1 RLC was shown to interact with PCNA, particularly when it is SUMOylated, and plays a role in unloading it from chromatin. 20,21

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Role of PCNA and RFC in promoting Mus81-complex activity

Oct 02, 2017 · PCNA interaction with the Mus81 complex and its effect on the Mus81 complex nuclease activity. The Mus81 complex has been suggested to play a role in processing stalled RFs [].We therefore wanted to test whether components of replication machinery can interact with the Mus81 complex and affect its enzymatic activity.

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The Yeast PCNA Unloader Elg1 RFC-Like Complex Plays a Role

The Elg1 Replication Factor C-like Complex Functions in

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Regulation of PCNA cycling on replicating DNA by RFC and

Jun 03, 2019 · Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to

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A role for the yeast PCNA unloader Elg1 in eliciting the

The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the unloading of PCNA during DNA damage.

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-tion at the site of DNA damage. A. mRad9 +/+ and mRad9

The yeast Elg1 RFC-like complex (RLC) is the principal unloader of chromatin-bound PCNA and thus plays a central role in maintaining genome stability. Here we identify a role for Elg1 in the

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Regulation of Elg1 activity by phosphorylation

Elg1 encodes a protein that forms an RFC-like complex that unloads the replicative clamp, PCNA, from DNA, mainly in its SUMOylated form. We have identified 2 different regions in yeast Elg1 that undergo phosphorylation. Phosphorylation of one of them, S112, is dependent on the ATR yeast ortholog, Mec1, and probably is a direct target of this

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The PCNA unloader Elg1 promotes recombination at collapsed

May 31, 2019 · To investigate whether RTS1-induced recombination depends on the PCNA unloader Elg1, we compared recombination frequencies in wild-type and elg1∆ strains harbouring either RTS1-IO or -AO (Figure 1C,D).In line with the observation that an elg1∆ mutant exhibits increased spontaneous direct repeat recombination in budding yeast (Gazy et al., 2013), a …

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